FastQCFastQC Report
Fri 1 Feb 2019
HHLCKBGX9_n02_KO_DI244_F1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHLCKBGX9_n02_KO_DI244_F1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17171466
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA292200.015.9979832
TAGGGGG78500.013.43098250-51
GGGGGAG284700.013.0931463
AAAGGGG227100.012.34746180-81
AAAAGGG222250.012.08104578-79
AAGGGGG219950.011.83049580-81
GGGGAGA220300.011.5954234
TATAGGG38500.010.73180748-49
AAAAAGG283050.010.72001878-79
ACATCTA108250.010.3953534
CGGGGAT54350.010.3206611
AAAAAAG395100.010.1449276-77
CGGGAAT39400.010.0139861
TAAAAAA441500.09.87840870-71
CGGCGAT23000.09.7139321
AGGGGGG330850.09.68969382-83
GGGGGCG170900.09.6022656-57
CCGGAAT40900.09.53049851
GGGAAAA220150.09.44677822-23
GGCGAAT24200.09.428681
Produced by FastQC (version 0.11.5)