FastQCFastQC Report
Fri 1 Feb 2019
HHLCKBGX9_n02_KO_Control_M.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHLCKBGX9_n02_KO_Control_M.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17646504
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATCTA117350.011.5315394
ACCTTAC57100.011.2251886
GGGGGGA240300.010.8533352
AACCTTA67900.010.3486325
CGGGAAT39700.010.177021
AAGGGGG182050.010.03800580-81
AAAAGGG184100.09.93809378-79
TAGGGGG69500.09.77175950-51
ATCTACA122350.09.662546
GCGGAAT26600.09.649491
CGACGAT19200.09.4075091
CGGCAAT27300.09.2279551
CGGGGAT58900.09.1191721
CATCTAC131450.09.1018775
CTCGAAT44300.09.0129671
TAGCGAT26100.08.9139719
CCGATAT25300.08.830181
CCCACAT146050.08.7547341
CGGAAAT45300.08.7090771
CCGGGAT54650.08.6976531
Produced by FastQC (version 0.11.5)