FastQCFastQC Report
Fri 1 Feb 2019
HHLCKBGX9_n02_KO_Control_F.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHLCKBGX9_n02_KO_Control_F.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17283551
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA238550.011.709532
CGCGAAT11750.011.3273511
AAAAGGG192200.010.47090778-79
AAGGGGG188000.010.09879880-81
GCGGAAT24800.09.9668951
CGCCGAT25400.09.7314561
TAGGGGG70300.09.69451950-51
CGGGAAT39550.09.6150531
GGGGGAG258350.09.1900333
GGCGTAT20800.09.1412351
AAAGGGG198650.09.114799580-81
ACCTTAC53200.09.0140786
CGGAAAT41850.08.8594621
CCGGGAT50200.08.8061771
GCGAATA11403.4015102E-108.7509662
CGACGAT21900.08.6820861
CGAATCG9902.1565938E-88.6336183
CGCGGAT17650.08.6181381
ACATCTA103350.08.4532784
GGCGAAT22000.08.4265561
Produced by FastQC (version 0.11.5)