FastQCFastQC Report
Fri 1 Feb 2019
HHLCKBGX9_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHLCKBGX9_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30713690
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG356802711.617057409904183No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC257200.031.5153838
TCTCGTA67450.029.89819742-43
TATCTCG27550.028.96991540-41
ATGCCGT77500.027.95522948-49
CGGAAGA306650.027.2015484
TCGGAAG313700.026.651083
CTCGTAT64250.026.39562644-45
ATCGGAA320900.026.1735732
CGTCTGA152400.025.4727416-17
GATCGGA328300.025.1482751
ACGTCTG161600.024.44838714-15
ACACGTC168150.023.5806812-13
CACGTCT166400.023.4296114-15
GAACTCC167700.023.1915720-21
ATCTCGT73100.022.6482542-43
GCTTGAA99250.022.25624560-61
CACACGT177900.022.1683412-13
GCGAAAT18850.021.79902336-37
CTGAACT184850.021.68018318-19
CGCGAAA16600.021.31966836-37
Produced by FastQC (version 0.11.5)