FastQCFastQC Report
Fri 1 Feb 2019
HHLCKBGX9_n01_WT_PBS_M2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHLCKBGX9_n01_WT_PBS_M2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18146425
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT1023240.5638796622475226TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG136400.040.46562648-49
TAATGCG145000.039.5031134-35
TCGTATG138400.038.9184344-45
CTCGTAT133450.038.7247544-45
CGTATGC142250.038.5165946-47
AATGCGC154850.037.1436834-35
TCTCGTA142050.035.92985542-43
TGCGCAT163700.033.75931536-37
ATCTCGT152900.033.3646942-43
ATGCGCA171200.033.1820636-37
ATGCCGT167250.033.04416348-49
GCGCATC164950.032.0358938-39
CTAATGC182550.031.68964832-33
TGCCGTC173500.031.42980450-51
CGCATCT166050.031.26586238-39
GCCGTCT171250.031.1631850-51
GTATGCC180550.030.96431546-47
CCGTCTT174600.030.64725552-53
ACTAATG190200.030.48997932-33
CATCTCG182450.028.16945640-41
Produced by FastQC (version 0.11.5)