FastQCFastQC Report
Fri 1 Feb 2019
HHLCKBGX9_n01_WT_PBS_M1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHLCKBGX9_n01_WT_PBS_M1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15225809
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT755530.4962166542349244TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG100100.039.5523448-49
CTCGTAT99750.038.30731644-45
TATCTCG96800.038.15242440-41
TCGTATG104500.038.13416744-45
CGTATGC104850.037.87196446-47
TCTCGTA107200.034.6725642-43
ATCTCGT113900.032.8415242-43
ATGCCGT124250.031.96027848-49
TGCCGTC132200.030.0389550-51
GCCGTCT133750.029.63756450-51
GTATGCC135650.029.62310446-47
CCGTCTT134550.029.3023852-53
AGCTATC137550.027.3672738-39
GCTATCT139850.026.83227238-39
CTATCTC140600.026.5880540-41
AAGCTAT157200.025.09367236-37
CGTCTTC162700.024.52450452-53
GCTTGAA168850.023.79801460-61
AGGGGGG156250.022.42909674-75
GAAGCTA182300.022.3421336-37
Produced by FastQC (version 0.11.5)