FastQCFastQC Report
Fri 1 Feb 2019
HHLCKBGX9_n01_WT_PBS_F2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHLCKBGX9_n01_WT_PBS_F2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20000078
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT1031090.5155429893823414TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA141400.041.1499936-37
TATGCCG140850.040.4006648-49
TCGTATG146450.038.87071244-45
GCGAAAT150000.038.80655736-37
CGTATGC148750.038.28579346-47
CTCGTAT143200.038.11104644-45
CCGCGAA158400.036.77700834-35
TCCGCGA162200.035.9886134-35
CGAAATC154650.035.65966838-39
TCTCGTA152800.035.62533642-43
AATCTCG153200.035.62529440-41
ATGCCGT172000.033.24960748-49
ATCTCGT165300.032.94571342-43
ACTCCGC183700.031.93092732-33
TGCCGTC178600.031.91455850-51
GCCGTCT179200.031.14504250-51
GTATGCC184800.031.07418646-47
CACTCCG196550.029.93791830-31
CCGTCTT186700.029.93221352-53
CTCCGCG195850.029.78026232-33
Produced by FastQC (version 0.11.5)