FastQCFastQC Report
Fri 1 Feb 2019
HHLCKBGX9_n01_WT_PBS_F1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHLCKBGX9_n01_WT_PBS_F1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17540116
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT1078320.6147735853058213TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG154750.040.88425448-49
TCGTATG160050.039.75370844-45
CGTATGC161500.039.35216546-47
CTCGTAT148400.039.04959544-45
TCTCGTA153250.037.1326242-43
ATGCCGT182000.035.14127348-49
ATCTCGT162200.034.4247742-43
CGGCTAT190550.033.35041434-35
GCCGTCT190700.033.2764150-51
TGCCGTC191450.033.20807350-51
GTATGCC192150.033.18630646-47
CCGTCTT193850.032.55246452-53
CCGGCTA198050.032.2908432-33
GATCTCG176700.031.70753940-41
TATGATC179450.031.44595738-39
ACCGGCT221800.028.7047132-33
TCACCGG222200.028.66216930-31
CTATGAT221000.028.1011836-37
CGTCTTC229100.027.69934552-53
GCTTGAA239700.026.76126360-61
Produced by FastQC (version 0.11.5)