FastQCFastQC Report
Fri 1 Feb 2019
HHLCKBGX9_n01_WT_DI244_M2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHLCKBGX9_n01_WT_DI244_M2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17169986
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT940050.5474960783311064TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG127300.040.1296548-49
CGTATGC128450.039.5293246-47
TAATGCG133250.039.4764234-35
TCGTATG128350.038.85682744-45
CTCGTAT125400.038.31265344-45
AATGCGC139750.037.58932534-35
TCTCGTA132600.035.7678642-43
TGCGCAT152800.033.66488336-37
ATGCCGT151850.033.57922448-49
ATCTCGT140750.033.49427442-43
ATGCGCA156200.033.46424536-37
CGCATCT151100.031.84419338-39
TGCCGTC158400.031.80058350-51
GCGCATC153650.031.76395438-39
CTAATGC168000.031.59325832-33
GCCGTCT157300.031.52472950-51
GTATGCC165450.031.16297146-47
ACTAATG174700.030.53114132-33
CCGTCTT164350.030.2018252-53
CACTAAT188250.028.4202830-31
Produced by FastQC (version 0.11.5)