FastQCFastQC Report
Fri 1 Feb 2019
HHLCKBGX9_n01_WT_DI244_M1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHLCKBGX9_n01_WT_DI244_M1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18451221
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT987200.5350323428460371TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG132250.040.22767648-49
TCGTATG135100.039.2900944-45
CTCGTAT131750.038.9371344-45
CGTATGC136750.038.868646-47
TATCTCG134650.037.49949340-41
TCTCGTA140200.036.1161842-43
ATCTCGT148700.034.1155942-43
ATGCCGT157950.033.63713548-49
GCCGTCT168300.031.45564550-51
TGCCGTC170250.031.2348650-51
GTATGCC173500.030.75882346-47
CCGTCTT173700.030.60114152-53
AGCTATC177350.028.97962838-39
CTATCTC175600.028.97099740-41
GCTATCT179100.028.4577738-39
AAGCTAT202900.026.20772636-37
CGTCTTC210300.025.54645552-53
AGGGGGG203650.023.7423374-75
GAAGCTA229500.023.69791436-37
GCTTGAA229150.023.52605860-61
Produced by FastQC (version 0.11.5)