FastQCFastQC Report
Fri 1 Feb 2019
HHLCKBGX9_n01_WT_DI244_F2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHLCKBGX9_n01_WT_DI244_F2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17219202
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT877160.5094080434157169TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA122200.041.35770836-37
TATGCCG123950.040.04697448-49
TCGTATG128100.038.85975644-45
CGTATGC128950.038.54863446-47
GCGAAAT132050.038.3626436-37
CTCGTAT122900.038.1850244-45
CCGCGAA136150.037.39710234-35
CGAAATC130850.036.64623338-39
TCCGCGA140000.036.36868334-35
TCTCGTA134600.035.18459742-43
AATCTCG134950.035.02289240-41
ATGCCGT148300.033.74374848-49
ATCTCGT141700.033.2205142-43
ACTCCGC159200.032.16137732-33
GCCGTCT155550.031.6978550-51
TGCCGTC156950.031.62695950-51
GTATGCC162300.031.03724946-47
CCGTCTT159600.030.72992552-53
CACTCCG169900.030.27388430-31
CTCCGCG172250.029.93157432-33
Produced by FastQC (version 0.11.5)