FastQCFastQC Report
Fri 1 Feb 2019
HHLCKBGX9_n01_WT_DI244_F1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHLCKBGX9_n01_WT_DI244_F1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17460218
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT816930.46788075612801633TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG116550.039.2264348-49
TCGTATG119550.038.36071844-45
CTCGTAT111900.037.92700244-45
CGTATGC121000.037.8029446-47
TCTCGTA120900.034.2598542-43
ATGCCGT142300.032.4619848-49
CGGCTAT145450.031.43103234-35
ATCTCGT130450.031.26018142-43
TGCCGTC152800.030.16919550-51
CCGGCTA154050.029.78414732-33
GCCGTCT155300.029.69882850-51
GTATGCC157000.029.42216746-47
CCGTCTT157800.028.98787752-53
GATCTCG143750.028.66520340-41
TATGATC148000.027.8743438-39
TCACCGG174250.026.41182130-31
ACCGGCT175750.026.13370132-33
CGTCTTC185850.025.03450452-53
CTATGAT183700.024.34435836-37
GTCACCG192550.024.05948628-29
Produced by FastQC (version 0.11.5)