FastQCFastQC Report
Fri 1 Feb 2019
HHLCKBGX9_n01_WT_Control_M.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHLCKBGX9_n01_WT_Control_M.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15177899
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT700060.46123643331662706TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG97400.040.0133848-49
TCGTATG101550.038.40149344-45
CGTATGC102950.038.24800546-47
CTCGTAT96150.038.03875744-45
TCTCGTA103450.034.4137342-43
CGGCTAT116550.033.6399534-35
ATCTCGT108650.032.3950842-43
ATGCCGT122900.031.82711648-49
CCGGCTA124750.031.485832-33
TGCCGTC128850.030.4309950-51
GTATGCC130700.030.0727446-47
GCCGTCT129900.030.05703250-51
TATGATC119850.029.38757138-39
GATCTCG119400.029.37927440-41
TCACCGG134700.029.22870330-31
CCGTCTT134450.029.07542452-53
ACCGGCT138650.028.48342332-33
GTCACCG150350.026.24676728-29
CACCGGC153300.025.62040330-31
CTATGAT150500.025.20098936-37
Produced by FastQC (version 0.11.5)