FastQCFastQC Report
Fri 1 Feb 2019
HHLCKBGX9_n01_WT_Control_F.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHLCKBGX9_n01_WT_Control_F.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15920565
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT721430.4531434656998668TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA96500.040.9752736-37
TATGCCG98200.039.6629548-49
TCGTATG103200.037.74028444-45
GCGAAAT104750.037.7254336-37
CTCGTAT98500.037.32293744-45
CGTATGC106000.036.7437846-47
CCGCGAA111400.035.77080534-35
CGAAATC106000.035.30987538-39
TCTCGTA107200.034.33952742-43
TCCGCGA116150.034.30794534-35
AATCTCG109450.033.65503740-41
ATCTCGT118400.031.13131542-43
ATGCCGT125400.031.1166448-49
ACTCCGC129350.030.7696632-33
GTATGCC133250.029.51473846-47
TGCCGTC131650.029.45881350-51
GCCGTCT130300.029.29013650-51
CACTCCG138100.029.06001730-31
CCGTCTT134700.028.5275852-53
CTCCGCG147650.027.22943532-33
Produced by FastQC (version 0.11.5)