FastQCFastQC Report
Fri 1 Feb 2019
HHLCKBGX9_n01_KO_PBS_M2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHLCKBGX9_n01_KO_PBS_M2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17745259
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT1242600.7002433720465844TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG158000.041.92220748-49
TCGTATG159600.040.57802244-45
TAATGCG170050.040.5134634-35
CTCGTAT157350.039.86027544-45
CGTATGC166000.039.75800746-47
AATGCGC176250.038.8727234-35
TCTCGTA164100.037.57124342-43
ATCTCGT172800.035.81707842-43
ATGCCGT184250.035.71755648-49
ATGCGCA192850.035.45497536-37
TGCGCAT188000.035.38428536-37
GCGCATC188850.033.96872738-39
GCCGTCT189600.033.89555750-51
GTATGCC196800.033.82533646-47
TGCCGTC193900.033.73179250-51
CGCATCT187100.033.4105638-39
CTAATGC208000.033.07570332-33
ACTAATG210500.032.99876432-33
CCGTCTT196500.032.8870952-53
CATCTCG206450.030.1408640-41
Produced by FastQC (version 0.11.5)