FastQCFastQC Report
Fri 1 Feb 2019
HHLCKBGX9_n01_KO_PBS_M1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHLCKBGX9_n01_KO_PBS_M1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19629822
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT1249110.6363328205421322TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG161850.040.30994848-49
TATCTCG155150.039.6007840-41
TCGTATG168600.039.1020944-45
CTCGTAT161500.038.68889644-45
CGTATGC169250.038.67241746-47
TCTCGTA169750.036.53050242-43
ATGCCGT190250.034.52977848-49
ATCTCGT180600.034.0465342-43
TGCCGTC199500.032.7381350-51
GCCGTCT200050.032.7074850-51
GTATGCC204500.032.4012446-47
CCGTCTT204800.031.64781452-53
CTATCTC202300.030.44147940-41
GCTATCT203950.030.34623338-39
AGCTATC205800.030.11960238-39
AAGCTAT235900.027.76592336-37
CGTCTTC245000.026.85244452-53
AGGGGGG236550.025.51373974-75
GCTTGAA262350.025.14711260-61
GAAGCTA267100.024.91380536-37
Produced by FastQC (version 0.11.5)