FastQCFastQC Report
Fri 1 Feb 2019
HHLCKBGX9_n01_KO_PBS_F2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHLCKBGX9_n01_KO_PBS_F2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16384772
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT872740.5326531244987724TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA117150.040.82987236-37
TATGCCG118800.039.8631448-49
GCGAAAT124250.038.59230836-37
TCGTATG122450.038.49892844-45
CGTATGC122300.038.48859446-47
CTCGTAT117000.038.3842144-45
CCGCGAA130100.037.1093334-35
TCTCGTA126350.035.6584842-43
TCCGCGA134750.035.6525134-35
CGAAATC128350.035.5654538-39
AATCTCG127150.035.22876740-41
ATGCCGT139550.033.9698448-49
ATCTCGT132300.033.8752842-43
GCCGTCT146150.031.83433550-51
TGCCGTC148950.031.61858250-51
ACTCCGC152350.031.54886832-33
GTATGCC153350.031.0207346-47
CACTCCG158200.030.57551630-31
CCGTCTT155650.029.92254652-53
CTCCGCG163700.029.65158832-33
Produced by FastQC (version 0.11.5)