FastQCFastQC Report
Fri 1 Feb 2019
HHLCKBGX9_n01_KO_PBS_F1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHLCKBGX9_n01_KO_PBS_F1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15182697
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT942240.6206012014861391TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG127550.041.15042548-49
CGTATGC132850.039.526146-47
TCGTATG133650.039.25396344-45
CTCGTAT125450.038.9611344-45
TCTCGTA129300.036.7550342-43
ATGCCGT151900.034.6164448-49
CGGCTAT152650.034.50660334-35
ATCTCGT138950.033.8947542-43
TGCCGTC156800.033.3680650-51
CCGGCTA158700.033.32526832-33
GTATGCC160050.033.03134546-47
GCCGTCT159450.032.84328550-51
CCGTCTT165950.031.64273352-53
TATGATC150200.031.577438-39
GATCTCG149600.031.51364140-41
TCACCGG174400.030.30941430-31
ACCGGCT176350.030.09763532-33
CTATGAT183450.027.82117536-37
CACCGGC191550.027.63291730-31
GTCACCG191450.027.60736828-29
Produced by FastQC (version 0.11.5)