FastQCFastQC Report
Fri 1 Feb 2019
HHLCKBGX9_n01_KO_DI244_F2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHLCKBGX9_n01_KO_DI244_F2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18233659
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT1758290.9643100158887472TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG214600.043.26144848-49
TCGTATG222150.042.121244-45
CTCGTAT213550.042.0825844-45
TCGTATC211100.041.8288738-39
CGTATGC224350.041.52831346-47
TATCTCG213600.041.37288340-41
CGTATCT215950.040.7904638-39
TTCGTAT225650.040.78330636-37
AATTCGT229600.040.76217734-35
ATTCGTA232400.040.14037336-37
TCTCGTA222550.040.02930542-43
ACGAATT238800.039.61841632-33
GAATTCG239750.039.1751534-35
CGAATTC244100.038.60255432-33
ATGCCGT243450.038.28109448-49
ATCTCGT233550.038.0422742-43
CACGAAT250350.037.89245230-31
GCCGTCT247350.037.39854450-51
TGCCGTC250250.037.0315950-51
CCGTCTT252750.036.47807352-53
Produced by FastQC (version 0.11.5)