FastQCFastQC Report
Fri 1 Feb 2019
HHLCKBGX9_n01_KO_DI244_F1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHLCKBGX9_n01_KO_DI244_F1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17171466
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT1554540.9053041831140103TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG191150.042.61747448-49
CTCGTAT189500.041.34520744-45
TCGTATC187250.041.33480538-39
CGTATGC198600.041.2693846-47
TATCTCG186900.041.03105540-41
TCGTATG200000.040.83703644-45
AATTCGT203950.040.2779134-35
TTCGTAT201900.039.9585236-37
CGTATCT193050.039.68696238-39
ATTCGTA210950.039.0323336-37
ACGAATT212650.038.9312432-33
TCTCGTA198150.038.86982342-43
GAATTCG215000.038.45081734-35
ATGCCGT214250.038.0225548-49
CGAATTC217500.037.82290332-33
ATCTCGT206650.037.51236342-43
CACGAAT223150.036.8942830-31
TGCCGTC224400.036.31266850-51
GCCGTCT225050.035.96506550-51
GTCACGA230050.035.87714428-29
Produced by FastQC (version 0.11.5)