FastQCFastQC Report
Fri 1 Feb 2019
HHLCKBGX9_n01_KO_Control_M.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHLCKBGX9_n01_KO_Control_M.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17646504
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT928910.5263988833142248TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG125900.040.29267548-49
TATCTCG121700.039.38004740-41
CTCGTAT126250.038.88216444-45
TCGTATG133000.038.10518644-45
CGTATGC137450.037.01000246-47
TCTCGTA134700.035.73827742-43
ATGCCGT151250.033.69650748-49
ATCTCGT147650.032.76461442-43
TGCCGTC160650.031.88692350-51
GCCGTCT160100.031.81845750-51
GTATGCC160550.031.74415846-47
CCGTCTT168700.030.14061752-53
CTATCTC163200.029.6862740-41
GCTATCT165100.029.18665138-39
AGCTATC170400.028.557638-39
AAGCTAT193550.026.14754736-37
CGTCTTC204800.024.966952-53
GCTTGAA213950.023.75379460-61
GAAGCTA220150.023.48445136-37
AGGGGGG197800.023.31676974-75
Produced by FastQC (version 0.11.5)