FastQCFastQC Report
Fri 1 Feb 2019
HHLCKBGX9_n01_KO_Control_F.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHLCKBGX9_n01_KO_Control_F.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17283551
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT985750.5703399723818329TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG129200.040.64295248-49
TAATGCG138600.039.374534-35
TCGTATG133350.039.07470744-45
CTCGTAT130450.038.41408544-45
CGTATGC137800.038.10612546-47
AATGCGC148100.036.7846534-35
TCTCGTA133700.036.7003242-43
TGCGCAT155350.033.98489836-37
ATGCGCA159700.033.95148536-37
ATCTCGT145550.033.85921542-43
ATGCCGT154850.033.8186648-49
GCGCATC157200.032.02938538-39
CGCATCT155400.031.95717238-39
TGCCGTC165200.031.55625250-51
GTATGCC169100.031.37580146-47
CCGTCTT165250.031.24484352-53
CTAATGC177050.031.14526432-33
GCCGTCT165950.030.9556750-51
ACTAATG180550.030.72564932-33
CATCTCG177350.027.81494140-41
Produced by FastQC (version 0.11.5)