FastQCFastQC Report
Wed 14 Oct 2020
HHL37AFX2_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL37AFX2_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9586500
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG133832113.960475668909403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGGG26100.011.959918130-134
CCCCCGG19500.010.498198130-134
CCCTGGG21300.010.156333130-134
CCCCTGG14300.09.848407130-134
CCCGGGG54550.09.182366130-134
CCCCCCG21100.08.737607125-129
CGACCCG8457.6319097E-48.57999145
CCCCCCT17600.07.5058513125-129
TCGCCGG24205.2386895E-107.190174145
GCCCCCC22500.06.8356385120-124
CCCCCTG13600.06.7256136130-134
ACCCGGG10200.06.6900287130-134
CCTGGGG47500.06.4493966130-134
TCCCCCC18950.06.4348927125-129
AATGCCC15850.00140498075.946448145
CCCAGGG17150.05.9260345130-134
ACCCCCG7153.1650416E-105.8879375125-129
CTCAACC17557.5151195E-45.783549145
GACCCCC10400.05.722974125-129
TAGATCG82800.05.691497145
Produced by FastQC (version 0.11.5)