FastQCFastQC Report
Wed 14 Oct 2020
HHL37AFX2_n02_AH7797-3H-input-rep.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL37AFX2_n02_AH7797-3H-input-rep.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8052934
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG340800.4231997927711813No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG26900.022.900581145
TCGTGTT79200.021.961699145
GCGTCGG18750.020.872398145
TCGCCGG35600.017.50767145
GTAGGGG41150.015.850849145
GAGTGTT64500.015.618343145
CGGTGGG34650.015.477768145
AGAGCGG47450.011.913469145
GTCGTGG75700.010.722646145
GTCGCCC23153.6379788E-128.139584145
GTAGATA127400.07.9072456145
AGTGTAT80450.07.657249145
GTGGTCG216750.06.3194976145
CGCCGTT42000.06.212023145
GGTGGTG141300.06.154871145
GTGTAGG637400.06.0830526145
GGTCGCC192700.05.82948145
GATCTCG263950.05.7385807145
CTCGGTG227800.05.726624145
ATCTCGG253900.05.7088304145
Produced by FastQC (version 0.11.5)