FastQCFastQC Report
Wed 14 Oct 2020
HHL37AFX2_n02_AH7797-3H-Hop1MLChip-rep.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL37AFX2_n02_AH7797-3H-Hop1MLChip-rep.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5129111
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG461930.9006044127335127No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG15900.023.24636145
GCGTCGG12100.020.36458145
TCGTGTT44100.013.804562145
AGAGCGG30000.013.52847145
TCGCCGG14700.013.311542145
GTAGGGG28650.010.877417145
GAGTGTT42200.09.617396145
GTCGTGG52850.09.599198145
CGGTGGG24300.09.245648145
GTCGCCC11150.00146215557.149896145
GTGTAGG175800.07.049514145
GGTCGCC40800.06.9276567145
GGGGGAA47550.06.862577455-59
AGCGTCG262750.06.7026334145
ATCTCGG62100.06.6521983145
CTCGGTG53450.06.64401145
GGGCTCC12150.00364536196.5557977
GATCTCG70950.06.4353185145
GTGTAGA114000.06.4209375145
TGGTCGC47450.06.1095145
Produced by FastQC (version 0.11.5)