FastQCFastQC Report
Wed 14 Oct 2020
HHL37AFX2_n02_AH10905B-input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL37AFX2_n02_AH10905B-input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8289255
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG200710.2421327368985512No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG24200.028.749073145
TCTCGGG40150.025.270306145
TCGTGTT91200.024.077744145
GTAGGGG50650.023.179472145
CGGTGGG46100.022.480352145
TCGCCGG39700.021.358135145
AGAGCGG56900.018.977629145
GAGTGTT80850.018.46525145
GTCGTGG121850.011.12203145
CGCCGGG15252.6375346E-109.504476145
CGCGTAG9904.333836E-58.7761184
CGGAAGC59050.08.222859145
TGGTCGC171400.08.20274145
CGCCGTT44250.08.188884145
GGTCGCC157800.07.9911675145
GTAGATG93450.07.9877763145
GTGGTCG188150.07.8961906145
GGTGGTG142950.07.756669145
TCGGTGG244600.07.4664145145
CTCGGTG216800.07.0867085145
Produced by FastQC (version 0.11.5)