FastQCFastQC Report
Wed 14 Oct 2020
HHL37AFX2_n02_AH10905B-Red1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL37AFX2_n02_AH10905B-Red1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7599790
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG468980.6170959987052274No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG18950.017.210648145
TCGTGTT63050.014.368744145
TCTCGGG23300.013.064333145
TCGCCGG18700.011.239586145
GTCGTGG52450.08.981766145
AGAGCGG50450.08.906856145
GAGTGTT58250.08.585134145
GTAGGGG36800.08.468657145
GTGTAGG222250.08.348185145
GGTCGCC39100.08.341222145
GTGGTCG58200.08.094393145
CGGTGGG33700.07.957299145
TGTAGGG213400.07.845335145
TAGGGAA220250.07.667149145
CTCGGTG62400.07.665725145
AGCGTCG334500.07.3450956145
CGTCGTG312000.07.154677145
GTAGGGA195900.07.0599174140-144
CGTGTAG229500.07.030693140-144
TGGTCGC54200.06.9534116145
Produced by FastQC (version 0.11.5)