FastQCFastQC Report
Wed 14 Oct 2020
HHL37AFX2_n02_AH10905A-input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL37AFX2_n02_AH10905A-input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9375355
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG452930.4831070396800975No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG39700.028.47708145
TCGTGTT96900.024.007261145
GCGTCGG22800.022.885427145
CGGTGGG48450.021.240068145
GTAGGGG51900.018.152538145
TCGCCGG43850.017.35326145
GAGTGTT84950.016.464756145
AGAGCGG55450.013.984393145
CGCCGTT48350.011.391437145
GTCGTGG107000.09.820771145
AGTGTAT95400.08.584034145
GGTGGTG161900.08.504879145
GTAGATA144900.08.402379145
GTCGCCC25755.456968E-127.5988503145
CTCGGTG293750.07.4999275145
GTGGTCG264950.07.4398875145
TGGTCGC244550.07.3789244145
GTCGCCG227000.07.3428283145
CGTATCA181200.07.1590633145
GCCGTAT190100.07.128873145
Produced by FastQC (version 0.11.5)