FastQCFastQC Report
Wed 14 Oct 2020
HHL37AFX2_n02_AH10905A-Red1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL37AFX2_n02_AH10905A-Red1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7011274
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG785791.1207520915599647No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG17600.012.353653145
TCGTGTT54600.09.291636145
TAGATCG91750.07.820165145
CGTGTAG111900.07.7720923145
GAGCGTC181850.07.7317038145
AGCGTCG164900.07.6913786145
AGATCGG290450.07.6604414145
AAGAGCG219450.07.3316913145
TGTAGGG107350.07.2913594145
GATCGGA277400.07.2109145
CGTCGTG158850.07.117446145
GTGTAGG111800.07.0659657145
GTAGGGG37000.06.8557205145
TCGGAAG266900.06.734261145
AGAGCGG48250.06.6090975145
GCGTCGT154350.06.5838366140-144
CGGTGGG29751.5279511E-106.577542145
ATCGGAA289500.06.5590286145
GTCGTGT131400.06.354696140-144
TCGTGTA127200.06.302396140-144
Produced by FastQC (version 0.11.5)