FastQCFastQC Report
Wed 14 Oct 2020
HHL37AFX2_n02_AH10677-3H-input-rep.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL37AFX2_n02_AH10677-3H-input-rep.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8146902
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG233290.28635424852293545No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTT70750.018.13067145
GCGTCGG19400.014.569007145
TCGCCGG28100.014.442714145
TCTCGGG27650.014.153561145
GTAGGGG41000.012.019658145
GAGTGTT66350.011.687185145
CGGTGGG32900.011.454456145
AGAGCGG45450.010.364456145
GTCGTGG66650.08.807485145
GGTCGCC112000.06.470667145
GTGTAGG383200.06.449053145
TAGGGAA366400.06.1118135145
ATCTCGG154050.05.7864275145
GATCTCG161650.05.738539145
GAGCGTC573100.05.677838145
CGCCGTT43659.094947E-125.6449714145
CGTGTAG391050.05.55976145
GTAGGGA342700.05.5416093140-144
CGTCGTG516100.05.51856145
AAGAGCG640750.05.4968605145
Produced by FastQC (version 0.11.5)