FastQCFastQC Report
Wed 14 Oct 2020
HHL37AFX2_n02_AH10677-3H-Hop1MLChip-rep.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL37AFX2_n02_AH10677-3H-Hop1MLChip-rep.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7969343
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1281631.6082003246691727No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG33650.034.89073145
AGAGCGG63600.020.51141145
GATCGGG30250.015.812431145
CGGAAGC64450.014.505975145
CGGAAGG48600.014.315782145
GTAGGGG56200.013.9273205145
TCGTGTT81500.013.427632145
GTCGTGG136450.09.932262145
CGGTGGG46350.08.912617145
TCGCCGG23850.08.812308145
TCTCGGG27950.08.038222145
GTGTAGG192150.08.03377145
TGTAGGG176350.07.726139145
GGTCGCC40150.07.581303145
GAGCGTG35450.07.564245145
GATCTCG71500.07.3994074145
GAGTGTT79100.06.9633346145
CGTCGTG343150.06.927395145
AGCGTCG362500.06.817521145
TCGTGGG43200.06.710523145
Produced by FastQC (version 0.11.5)