FastQCFastQC Report
Wed 14 Oct 2020
HHL37AFX2_n02_AH10675-3H-input-rep.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL37AFX2_n02_AH10675-3H-input-rep.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7757921
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG293050.3777429545879624No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTT69600.016.86966145
TCGCCGG35150.016.083103145
TCTCGGG28550.015.485467145
GTAGGGG39150.014.06962145
GCGTCGG17950.013.324473145
CGGTGGG35950.012.701125145
GAGTGTT68800.012.219969145
AGAGCGG44450.010.109286145
GTCGTGG72850.08.655462145
AGTGTAT77500.07.2009463145
CGCCGTT38550.06.9562936145
CTCGGTG197450.06.4970574145
GTAGATA112900.06.162797145
GGTCGCC165300.06.050716145
ATCTCGG220700.05.746935145
GTGTAGG503750.05.697452145
TCGGTGG227550.05.6057844145
GGGGGAA59000.05.53060255-59
GTCGCCG166050.05.4559665145
GTCGCCC21302.6447402E-45.4442863145
Produced by FastQC (version 0.11.5)