FastQCFastQC Report
Wed 14 Oct 2020
HHL37AFX2_n02_AH10675-3H-Hop1MLChip-rep.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL37AFX2_n02_AH10675-3H-Hop1MLChip-rep.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6803205
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1087961.5991874418013272No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG16950.013.681429145
TCGTGTT50950.012.516696145
GCGTCGG16000.012.229113145
GGGGGAA114050.011.50801455-59
AAGGGGG127950.010.70157870-74
AGGGGGG261450.09.392570570-74
CGGTGGG30750.08.955496145
GGGGGTA49450.08.76903855-59
GGGGAAA132750.08.59779955-59
TCGCCGG17855.693437E-108.5257435145
GTCGTGG46050.08.497972145
GGGGTAA41150.08.24694555-59
GGGGGGA242950.07.527404355-59
GAGTGTT57600.07.4230213145
AAAGGGG113750.07.40868170-74
AGAGGGG75400.07.156286770-74
CTCGGTG70350.07.1078157145
GTGTAGG209750.06.840918145
GGGGAGA70950.06.80673655-59
GGGGGAT39450.06.690698655-59
Produced by FastQC (version 0.11.5)