FastQCFastQC Report
Wed 14 Oct 2020
HHL37AFX2_n02_AH10515-input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL37AFX2_n02_AH10515-input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9094295
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG325900.3583565301103604No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTT92500.028.206871145
TCTCGGG34300.027.469032145
TCGCCGG35350.020.297377145
GCGTCGG21550.020.178928145
CGGTGGG38750.019.82569145
GAGTGTT80500.017.736359145
GTAGGGG48250.016.82344145
AGAGCGG55250.016.003748145
GTCGTGG97950.011.098926145
CGCCGGG14801.537046E-99.30435145
GCCGTAT147300.08.807332145
CTCGGTG230050.08.695228145
GTGGTCG206750.08.693612145
AGTGTAT89000.08.550537145
GGTCGCC171800.08.479437145
CGTGTAT70200.08.465857145
TGGTCGC190350.08.33845145
GTAGATA144400.08.331727145
ATCTCGG267650.08.17775145
GATCTCG283250.07.932059145
Produced by FastQC (version 0.11.5)