FastQCFastQC Report
Wed 14 Oct 2020
HHL37AFX2_n02_AH10515-Red1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL37AFX2_n02_AH10515-Red1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7665756
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG758890.9899741134468669No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTT60900.011.186138145
GCGTCGG16750.010.384033145
GTAGGGG42050.07.7556133145
TCGCCGG18801.0912481E-77.3242865145
GTCGTGG45100.06.909737145
GGTCGCC44300.06.707331145
TGTAGGG183050.06.6117487145
AGCGTCG261050.06.6072826145
CTCGGTG62700.06.1260138145
AGATCGG412750.06.075173145
ATCGGAA409400.06.0186725145
AGAGCGG42150.06.017832145
GTGTAGG186200.05.993916145
GATCGGA394050.05.9588614145
GAGTGTT61400.05.90162145
ATCTCGG72900.05.8653526145
TAGGGAA194850.05.839409145
CGTGTAG187850.05.8033442140-144
TCGGTGG82450.05.8012676145
CGGAAGA383150.05.7879157145
Produced by FastQC (version 0.11.5)