FastQCFastQC Report
Wed 14 Oct 2020
HHL37AFX2_n02_AH10514-input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL37AFX2_n02_AH10514-input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10138077
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG864150.8523805846019911No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG38900.028.877869145
TCGTGTT101150.024.217752145
TCGCCGG47200.023.492674145
CGGTGGG46300.023.010147145
GAGTGTT88250.019.381186145
GCGTCGG26100.018.604471145
GTAGGGG52650.016.380669145
AGAGCGG61500.014.848358145
GTAGATA160450.010.343768145
GTCGTGG112600.09.5259075145
AGTGTAT103000.09.217585145
TGGTCGC281350.08.680925145
GTGGTCG301450.08.438686145
CTCGGTG340050.08.013608145
GGTCGCC258800.07.841096145
GTCGCCC25303.6379788E-127.7343936145
CCGTATA64600.07.6288557145
CGCCGTT49850.07.55999145
GTCGCCG253950.07.5056887145
CGTGTAT85100.07.494387145
Produced by FastQC (version 0.11.5)