FastQCFastQC Report
Wed 14 Oct 2020
HHL37AFX2_n02_AH10514-Red1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL37AFX2_n02_AH10514-Red1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9322397
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG398760.4277440662524885No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG22300.017.22332145
TCGTGTT75950.015.171035145
TCTCGGG28700.014.392585145
TCGCCGG22800.013.667196145
GTAGGGG38300.011.920312145
CGGTGGG34850.011.020947145
GTCGTGG61750.08.684416145
GGTCGCC68400.08.475781145
AGAGCGG52550.08.274169145
TGTAGGG301650.07.639582145
TAGATCG231150.07.4928985145
GAGTGTT70100.07.443211145
GTCGCCG75700.07.3712425145
GTGTAGG314400.07.2836723145
CTCGGTG94650.07.2735896145
AGCGTCG465150.07.2132974145
GAGCGTC498500.07.1377635145
AAGAGCG571250.06.7742453145
GCCGTAT70750.06.65783145
CGTGTAG326150.06.6435413145
Produced by FastQC (version 0.11.5)