FastQCFastQC Report
Wed 14 Oct 2020
HHL37AFX2_n02_AH10513-input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL37AFX2_n02_AH10513-input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9089401
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG243150.2675093771305722No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG36700.028.830894145
TCGTGTT95150.025.744192145
GCGTCGG25650.020.908154145
CGGTGGG52400.020.054323145
GAGTGTT83650.019.32014145
GTAGGGG50900.018.509594145
TCGCCGG40400.018.47682145
AGAGCGG59250.013.821697145
GTCGCCC25200.013.804224145
GTCGTGG107700.010.430073145
AGTGTAT93750.08.967225145
GGTCGCC220650.08.671042145
GTAGATA143150.08.657173145
CGCCGTT45700.08.40487145
TGGTCGC239600.08.28772145
GTCGCCG213800.08.135324145
GCCGTAT179700.08.106237145
CTCGGTG295350.08.097451145
ATCTCGG338500.08.071496145
GGTGGTG164250.07.765664145
Produced by FastQC (version 0.11.5)