FastQCFastQC Report
Wed 14 Oct 2020
HHL37AFX2_n02_AH10513-Red1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL37AFX2_n02_AH10513-Red1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7581308
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2982193.9336088178979143No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTT64300.018.372602145
TCTCGGG21700.018.035494145
GCGTCGG19600.015.90034145
TCGCCGG22100.013.117822145
GAGTGTT59700.012.50423145
AGAGCGG47450.011.30289145
GTAGGGG53400.010.857822145
CGGTGGG36250.010.79642145
GTCGTGG63700.010.353709145
GGGGGAA195300.09.25973855-59
AAGGGGG181350.09.18972270-74
TCGGTGG113050.08.654803145
GGGGGTA70550.08.53071855-59
GTCGCCG71750.08.282968145
CTCGGTG92700.08.209251145
GGGGTAA53350.08.18213455-59
GGTCGCC65700.08.052885145
GGGGAAA181350.07.972821755-59
ATCTCGG113300.07.9320564145
CGTGTAT39850.07.820493145
Produced by FastQC (version 0.11.5)