FastQCFastQC Report
Wed 14 Oct 2020
HHL37AFX2_n02_AH10512-input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL37AFX2_n02_AH10512-input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8898160
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG342560.38497846745844083No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG39900.028.698473145
TCGTGTT102350.026.270037145
TCGCCGG44850.023.430424145
GCGTCGG25350.022.871077145
GTAGGGG52800.021.412397145
CGGTGGG46750.019.532757145
AGAGCGG58100.018.336472145
GAGTGTT88250.018.066854145
GTCGTGG121750.011.309912145
CGCCGTT48750.010.555002145
CGCCGGG16601.364242E-109.168237145
GTAGATG99300.08.247148145
TGGTCGC251250.08.105407145
GTGGTCG270600.07.954324145
AGTGTAT87150.07.900068145
CGTATCA179350.07.7988486145
GTCGCCG233550.07.664638145
CTCGGTG304800.07.4897995145
GGTCGCC234800.07.4766293140-144
ATCTCGG348400.07.1557455145
Produced by FastQC (version 0.11.5)