FastQCFastQC Report
Wed 14 Oct 2020
HHL37AFX2_n02_AH10512-Red1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL37AFX2_n02_AH10512-Red1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7433546
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1149851.5468391532116705No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG21750.015.661459145
TCGTGTT62050.014.833908145
TCTCGGG23300.013.686437145
GTAGGGG39450.011.5741145
GTCGTGG56550.09.9966755145
AGAGCGG47500.09.917755145
GTGTAGG195000.08.659942145
CGTGTAG201750.07.7595015145
GAGTGTT58250.07.7141733145
TGGTCGC48050.07.6925516145
CGGTGGG35300.07.596625145
TGTAGGG189700.07.564697145
AGCGTCG302600.07.376926145
GGTCGCC35700.07.3084946145
AAGAGCG379050.07.0171895145
AGAGTGT143950.06.998368145
TCGCCGG18959.654013E-76.8842554145
GAGCGTC323400.06.790414145
TCGGAAG453450.06.7768846145
GTAGGGA173350.06.7403183140-144
Produced by FastQC (version 0.11.5)