FastQCFastQC Report
Wed 14 Oct 2020
HHL37AFX2_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL37AFX2_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9586500
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG133160.13890366661451No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC398050.022.0137988
GAGCACA426950.020.5910439
CACACGG24500.017.888016145
AAGAGCA513000.017.6836097
CGGAAGA526150.017.1305754
TCGGAAG530400.016.8983023
GATCGGG32250.016.760193145
ATCGGAA545850.016.4598272
GATCGGA549850.016.1263521
GAAGAGC577700.015.5022686
GGAAGAG614650.014.7226045
GAGCACC26500.012.954748145
CGGAAGG37800.011.980554145
ATGCCGT117200.011.63058445-49
CACGTCG24400.011.375508145
AAAGGGG103250.011.24295270-74
CTCGTAT121750.010.81803940-44
TCTCGTA125950.010.5031640-44
ATCTCGT132050.09.9195440-44
CGATCAG27750.09.89812730-34
Produced by FastQC (version 0.11.5)