FastQCFastQC Report
Wed 14 Oct 2020
HHL37AFX2_n01_AH7797-3H-input-rep.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL37AFX2_n01_AH7797-3H-input-rep.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8052934
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC448970.5575235063394286TruSeq Adapter, Index 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCACGC38000.021.368074145
ATGCCGG31550.021.140705145
GAGCACC41400.012.608492145
ACGATCG43350.011.874087145
ATCTCGG29250.011.6493845145
AGGGGGG102000.010.903254570-74
CACACGG28350.010.4848385145
CACGATG56700.010.356975145
AAGGGGG93800.010.07872870-74
TCACGAG51150.010.063376145
GCCGTCG47600.09.290815145
GAGCACA1010450.08.0430689
GCACACC36650.07.91256145
AGAGCAC1055750.07.6842238
AAAGGGG112100.07.24341570-74
CTGCTTG173150.07.184983355-59
AAGAGCA1176250.06.98947247
GAAGAGC1183050.06.9147626
GCCGTCT200350.06.868119245-49
GCTTGAA195000.06.840914755-59
Produced by FastQC (version 0.11.5)