FastQCFastQC Report
Wed 14 Oct 2020
HHL37AFX2_n01_AH7797-3H-Hop1MLChip-rep.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL37AFX2_n01_AH7797-3H-Hop1MLChip-rep.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5129111
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC116210.22656947763462326TruSeq Adapter, Index 3 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA372150.030.1177649
AGAGCAC393250.028.6308338
GAAGAGC452550.024.8391156
CGGAAGA455350.024.7023034
TCGGAAG457400.024.5123143
AAGAGCA459350.024.5108857
GGAAGAG478300.023.5321885
GATCGGA473050.023.5249711
ATCGGAA480400.023.3689372
AAGGGGG72650.018.70101770-74
AGGGGGG77850.018.62527370-74
AAAGGGG78450.016.65302370-74
TAGGCAT158950.013.69245835-39
CTTAGGC169700.013.20103330-34
CATCTCG50450.012.99087835-39
AGGCATC123100.012.8978535-39
ACTTAGG175000.012.84265830-34
TTAGGCA173600.012.745769530-34
CGGGGTG31050.012.56180250-54
GTATGCC45850.012.23863245-49
Produced by FastQC (version 0.11.5)