FastQCFastQC Report
Wed 14 Oct 2020
HHL37AFX2_n01_AH10905B-input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL37AFX2_n01_AH10905B-input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8289255
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATG60600.018.423811145
GAGCACC37600.012.340238145
CACACGG26300.010.750786145
CGGGGGG77550.09.535639145
GCACACC32200.08.105466145
GAATCGG191550.07.9860435145
TCGGGGG111400.07.939735145
GTCACGG21956.184564E-117.9269857145
ATCGGGG156100.07.8490157145
GCGGGGG42900.07.7737737145
TCGCGGG20951.5897967E-97.6132493145
GGGGGTG107300.07.567452145
AATCGGG176000.07.249889145
GGGGTGG117900.07.071563145
GGAATCG253300.06.7833548145
GTCACGT452950.06.7224936145
GTGGGGG79400.06.574207145
GTTTCGG362900.06.492736145
AGTCACG469900.06.4337177145
TCGGAAT333500.06.4129457145
Produced by FastQC (version 0.11.5)