FastQCFastQC Report
Wed 14 Oct 2020
HHL37AFX2_n01_AH10905B-Red1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL37AFX2_n01_AH10905B-Red1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7599790
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT584400.7689686162380802TruSeq Adapter, Index 16 (97% over 40bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA449800.022.6618129
GTCCCGC22100.021.323074145
AGAGCAC481550.021.2579848
CACCCGG16150.020.20081145
GAAGAGC570450.017.9401826
AAGAGCA580900.017.67227
CGGAAGA582950.017.5928234
TCGGAAG583400.017.5296383
GCCGTCT98150.017.3287250-54
GGAAGAG616600.016.7150615
GATCGGA611850.016.5203021
TGCCGTC113250.016.43938450-54
ATCGGAA625150.016.3128532
GTATGCC121050.016.24252945-49
CGTATGC121900.016.1530645-49
TATGCCG121600.016.03789945-49
ATGCCGT121650.015.64989445-49
CCCGATC117200.015.35334235-39
GTCCCGA129550.015.22159935-39
GATCTCG118050.015.18137940-44
Produced by FastQC (version 0.11.5)