FastQCFastQC Report
Wed 14 Oct 2020
HHL37AFX2_n01_AH10905A-input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL37AFX2_n01_AH10905A-input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9375355
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT360930.3849774221882798TruSeq Adapter, Index 20 (97% over 44bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGCCG39700.019.722588145
GAGCACC41450.015.216869145
CCTTATG65750.014.003565145
ATGCCGG54500.013.834626145
CACACGG31900.013.408862145
ATCTCGG43500.012.333123145
GCACACC35900.011.510946145
AAGGGGG99850.010.83306570-74
CTTATCG165850.010.578656145
TGCCGTG47400.09.941813145
GCCGTCG50600.09.886196145
AAAGGGG120400.09.34535670-74
GTCACGG22900.08.864478145
ATCGCGG39550.08.615529145
AGGGGGG101200.08.46774270-74
CCAGTCC37150.08.196362145
GCCGTCT111950.07.978425550-54
TCTCGTT94600.07.8169913145
TTATCGG77050.07.4333577145
TGCCGTC146150.07.16306750-54
Produced by FastQC (version 0.11.5)