FastQCFastQC Report
Wed 14 Oct 2020
HHL37AFX2_n01_AH10905A-Red1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL37AFX2_n01_AH10905A-Red1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7011274
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGGGGGT119050.1697979568335227TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGGGGTT87720.12511278264121473TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA260900.039.236749
AGAGCAC279350.036.6972128
TCGGAAG343100.030.287913
CGGAAGA347550.029.9627064
GATCGGA351950.029.0209181
GAAGAGC358700.028.889816
ATCGGAA368850.028.1150862
AAGAGCA372900.027.7047887
GGAAGAG384250.027.0443275
ATCGGGG56600.014.88408140-44
CACATGT143550.014.6058730-34
GCGGGGT36750.014.51953950-54
TCACATG144750.014.31449430-34
ACATGTC152800.013.95891530-34
ATGTCAG148800.013.78846330-34
ATCGCGG34000.013.7748240-44
CAGTCAC154300.013.66346525-29
GTCACAT150850.013.64914325-29
AGGGGGG84500.013.59035575-79
TGTCAGA153850.013.59033335-39
Produced by FastQC (version 0.11.5)