FastQCFastQC Report
Wed 14 Oct 2020
HHL37AFX2_n01_AH10677-3H-input-rep.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL37AFX2_n01_AH10677-3H-input-rep.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8146902
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACC40800.09.77314145
CACACGG29100.08.719802145
AGGGGGG66000.07.447616670-74
GCGGGGG38800.07.287263145
AAGGGGG66800.07.27159970-74
TCGGGGG54000.06.847121145
GAGCACA620550.06.3321929
AAAGGGG82500.06.02839570-74
CAGTCAC320500.06.0170717145
AGAGCAC653050.05.99485838
GGCGGGG54150.05.890956145
GGGTGGG62350.05.8138676145
ACGTCTG485650.05.687654145
CACTAGC273400.05.648234145
GAACTCC397700.05.5964723145
AAGAGCC68600.05.495546145
CGTCTGA471800.05.4550905145
TGAACTC437350.05.420635145
CACGTCT505750.05.3756003145
ACACGTC524200.05.366193145