FastQCFastQC Report
Wed 14 Oct 2020
HHL37AFX2_n01_AH10677-3H-Hop1MLChip-rep.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL37AFX2_n01_AH10677-3H-Hop1MLChip-rep.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7969343
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC1134831.4239944246345024TruSeq Adapter, Index 11 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA568350.036.915829
AGAGCAC598100.035.0917178
GAAGAGC696700.030.3871426
AAGAGCA701950.030.158287
CGGAAGA700500.030.1498264
TCGGAAG703300.029.9885433
GGAAGAG733950.028.8152665
GATCGGA733250.028.4760231
ATCGGAA747100.028.2989042
AGGGGGG135750.020.89242670-74
GCCGTCT152250.019.5901145-49
TGCCGTC174650.019.21120645-49
AAGGGGG127950.019.10632370-74
ATGCCGT187500.018.61378745-49
GTATGCC200700.018.09034345-49
TATGCCG201600.017.90889245-49
CGTATGC205450.017.59446340-44
CCGTCTT176350.017.37336350-54
CATCTCG216050.017.30840335-39
CTCGTAT216250.016.89679340-44
Produced by FastQC (version 0.11.5)